General Protocols

Publications

  • Acharya, P., Shrestha, S., Joshi, P., Choi, N.Y., Lekkala, V.K.R., Kang, S.Y., Ni, G., and Lee, M.Y., Dynamic culture of cerebral organoids using a pillar/perfusion plate for the assessment of developmental neurotoxicity, Submitted for publication (2024)
  • Shrestha, S., Lekkala, V.K.R., Acharya, P., Kang, S.Y., Vanga, M.G., and Lee, M.Y., Reproducible generation of human liver organoids (HLOs) on a pillar plate platform via microarray 3D bioprinting, Submitted for publication (2024)
  • Acharya, P., Joshi, P., Shrestha, S., Choi, N.Y., Jeong, S.H., and Lee, M.Y., Uniform cerebral organoid culture on a pillar plate by simple and reproducible spheroid transfer from an ultralow attachment well plate, Biofabrication, doi.org/10.1088/1758-5090/ad1b1e (2024)
  • Acharya, P., Choi, N.Y., Shrestha, S., Jeong, S.H., and Lee, M.Y., Brain organoids: A revolutionary tool for modeling neurological disorders and development of therapeutics, Biotechnology and Bioengineering, doi.org/10.1002/bit.28606 (2023)
  • Joshi, P., Kang, S.Y., Acharya, P., Sidhpura, D., and Lee, M.Y., High-throughput assessment of metabolism-mediated neurotoxicity by combining 3D-cultured neural stem cells and liver cell spheroids, Toxicology In Vitro, DOI: 10.1016/j.tiv.2023.105688 (2023)
  • Kang, S.Y., Kimura, M., Shrestha, S., Lewis, P., Lee, S.J., Cai, Y., Joshi, P., Acharya, P., Liu, J., Yang, Y., Sanchez, J.G., Ayyagari, S., Alsberg, E., Wells, J.M., Takebe, T., and Lee, M.Y., A pillar and perfusion plate platform for robust human organoid culture and analysis, Advanced Healthcare Materials, DOI: 10.1002/adhm.202302502 (2023)
  • Lekkala, V.K.R., Kang, S.Y., Liu, J., Shrestha, S., Acharya, P., Joshi, P., Jamdagneya, P., Pagnis, S., Kundi, A., Kabbur, S., Zolfaghar, M., Lee, M.S., Kim, U.T., Yang, Y., and Lee, M.Y., A pillar/perfusion plate enhances cell growth, reproducibility, throughput, and user friendliness in dynamic 3D cell culture, BioRxiv, https://doi.org/10.1101/2023.02.16.528892 (2023)
  • Joshi, P., Patel, R., Kang, S.Y., Serbinowski, E., and Lee, M.Y., Establishment of ion channel and ABC transporter assays in 3D-cultrued ReNcell VM on a 384-pillar plate for neurotoxicity potential, Toxicology In Vitro, doi: 10.1016/j.tiv.2022.105375 (2022)
  • Shrestha, S., Lekkala, V.K.R., Acharya, P., Siddhpura, D., and Lee, M.Y., Recent advances in microarray 3D bioprinting for high-throughput spheroid and tissue culture and analysis, Essays in Biochemistry, doi: 10.1042/EBC20200150 (2021)
  • Kang, S.Y., Joshi, P., and Lee, M.Y., High-throughput screening of compound neurotoxicity using 3D-cultured neural stem cells on a 384-pillar plate, Current Protocols in Toxicology, doi.org/10.1002/cpz1.107 (2021)
  • Bigdelou, P., Chan, K.K., Tang, J.S., Yu, K.N., Whited, J., Wang, D., Lee, M.Y., and Sun, X.L., High-throughput multiplex assays with mouse macrophages on pillar plate platforms, Experimental Cell Research, 396, 112243, doi.org/10.1016/j.yexcr.2020.112243 (2020)
  • Joshi, P., Kang, S.Y., Yu, K.N., Kothapalli, C., and Lee, M.Y., High-content imaging of 3D-cultured neural stem cells on a 384-pillar plate for the assessment of cytotoxicity, Toxicology In Vitro, 65, 104765, DOI: 10.1016/j.tiv.2020.104765 (2020)
  • Yu, K.N., Kang, S.Y., Hong, S., and Lee, M.Y., High-throughput metabolism-induced toxicity assays demonstrated on a 384-pillar plate, Archives of Toxicology, 92, 2501-2516, DOI: 10.1007/s00204-018-2249-1 (2018)
  • Lee, M.Y., Microarray Bioprinting Technology: Fundamentals and Practices, Springer, ISBN # 978-3-319-46803-7 (2016)
  • Lee, M.Y., Perfusion plate for microarray 3D bioprinting, US 2021-0123007 (Issued in 2023)
  • Lee, M.Y., Chip platforms for microarray 3D bioprinting, WO 2018-094194, US20180142195, CN110087772, EP3541518, CA3043708, IN201937021421 (Issued in 2022)