Posters
General Protocols
Publications
- Shrestha, S., Acharya, P., Kang, S.Y., Vanga, M.G., Lekkala, V.K.R., Liu, J., Yang, Y., Joshi, P., and Lee, M.Y Regenerative human liver organoids (HLOs) in a pillar/perfusion plate for hepatotoxicity assays, BioRxiv, doi.org/10.1101/2024.03.25.586638 (2024)
Model: Liver organoids Application: Liver organoid regeneration and hepatotoxicity assays - Acharya, P., Shrestha, S., Joshi, P., Choi, N.Y., Lekkala, V.K.R., Kang, S.Y., Ni, G., and Lee, M.Y., Dynamic culture of cerebral organoids using a pillar/perfusion plate for the assessment of developmental neurotoxicity, BioRxiv, doi.org/10.1101/2024.03.11.584506 (2024)
Model: Brain organoids Application: Developmental neurotoxicity - Lekkala, V.K.R., Kang, S.Y., Liu, J., Shrestha, S., Acharya, P., Joshi, P., Zolfaghar, M., Lee, M.S., Vanga, M.G., Jamdagneya, P., Pagnis, S., Kundi, A., Kabbur, S., Kim, U.T., Yang, Y., and Lee, M.Y., A pillar/perfusion plate enhances cell growth, reproducibility, throughput, and user friendliness in dynamic 3D cell culture, ACS Biomaterials Science & Engineering, doi.org/10.1021/acsbiomaterials.4c00179 (2024)
Model: 3D cell culture Application: Dynamic spheroid culture for drug testing - Shrestha, S., Lekkala, V.K.R., Acharya, P., Kang, S.Y., Vanga, M.G., and Lee, M.Y., Reproducible generation of human liver organoids (HLOs) on a pillar plate platform via microarray 3D bioprinting, Lab on a chip, DOI: 10.1039/D4LC00149D (2024)
Model: Liver organoid Application: Hepatotoxicity - Acharya, P., Joshi, P., Shrestha, S., Choi, N.Y., Jeong, S.H., and Lee, M.Y., Uniform cerebral organoid culture on a pillar plate by simple and reproducible spheroid transfer from an ultralow attachment well plate, Biofabrication, doi.org/10.1088/1758-5090/ad1b1e (2024)
Model: Brain organoid Application: Developmental neurotoxicity - Kang, S.Y., Kimura, M., Shrestha, S., Lewis, P., Lee, S.J., Cai, Y., Joshi, P., Acharya, P., Liu, J., Yang, Y., Sanchez, J.G., Ayyagari, S., Alsberg, E., Wells, J.M., Takebe, T., and Lee, M.Y., A pillar and perfusion plate platform for robust human organoid culture and analysis, Advanced Healthcare Materials, DOI: 10.1002/adhm.202302502 (2023)
Model: Liver and intestine organoids Application: Pillar/perfusion plate for disease modeling - Joshi, P., Kang, S.Y., Acharya, P., Sidhpura, D., and Lee, M.Y., High-throughput assessment of metabolism-mediated neurotoxicity by combining 3D-cultured neural stem cells and liver cell spheroids, Toxicology In Vitro, DOI: 10.1016/j.tiv.2023.105688 (2023)
Model: Liver and brain co-culture Application: Metabolism-induced neurotoxicity - Joshi, P., Patel, R., Kang, S.Y., Serbinowski, E., and Lee, M.Y., Establishment of ion channel and ABC transporter assays in 3D-cultrued ReNcell VM on a 384-pillar plate for neurotoxicity potential, Toxicology In Vitro, DOI: 10.1016/j.tiv.2022.105375 (2022)
Model: 3D neural stem cell culture Application: Neurotoxicity - Kang, S.Y., Joshi, P., and Lee, M.Y., High-throughput screening of compound neurotoxicity using 3D-cultured neural stem cells on a 384-pillar plate, Current Protocols in Toxicology, doi.org/10.1002/cpz1.107 (2021)
Model: 3D neural stem cell culture Application: High-throughput screening - Bigdelou, P., Chan, K.K., Tang, J.S., Yu, K.N., Whited, J., Wang, D., Lee, M.Y., and Sun, X.L., High-throughput multiplex assays with mouse macrophages on pillar plate platforms, Experimental Cell Research, 396, 112243, doi.org/10.1016/j.yexcr.2020.112243 (2020)
Model: Mouse macrophages Application: High-throughput immunoassays - Joshi, P., Kang, S.Y., Yu, K.N., Kothapalli, C., and Lee, M.Y., High-content imaging of 3D-cultured neural stem cells on a 384-pillar plate for the assessment of cytotoxicity, Toxicology In Vitro, 65, 104765, DOI: 10.1016/j.tiv.2020.104765 (2020)
Model: 3D cell culture Application: Neurotoxicity - Yu, K.N., Kang, S.Y., Hong, S., and Lee, M.Y., High-throughput metabolism-induced toxicity assays demonstrated on a 384-pillar plate, Archives of Toxicology, 92, 2501-2516, DOI: 10.1007/s00204-018-2249-1 (2018)
Model: 3D bioprinting Application: Metabolism-induced hepatotoxicity